LIGHTNOVELONLINE Chapter 237 No money for crappy scientific research
After coming out of Ji Hong's office, Lu Liangliang already had a clear direction for drug development.
At first, his idea was to develop drugs based on the β-amyloid hypothesis, but Lu Liang had been hesitant because of whether the hypothesis was really true.
After all, the theory was wrong from the beginning, and the final result will definitely be wrong at grandma's house.
But it's different now. Lu Liang has already gotten the answer to this question from Ji Hong.
The hypothesis of β-amyloid protein can be established, and Biogen has developed drugs based on this hypothesis.
Although the side effects of the drug are fatal, it is undeniable that this drug has proven that it can have a certain therapeutic effect on Alzheimer's disease by clearing abnormal deposition of amyloid protein.
After Lu Liang returned to the study room, he turned on the system, and the panel appeared in front of Lu Liang.
[Level: lv6]
[Analysis target: β-amyloid antibody]
[Based on the β-amyloid hypothesis, an antibody can be used to clear abnormal deposition of β-amyloid protein outside nerve cells. 】
[Source points required for analysis: 4]
[Existing source point: 13.5]
[After analysis, a β-amyloid antibody can be obtained. 】
[Start parsing? 】
Lu Liang was a little surprised when he saw the number of source points in the system. He remembered that the last time he looked at it, there were only 10.2 source points in the system.
How come after coming back after the new year, the number of source points in the system has increased to 13.5.
Where did the extra 3.2 source points come from? Could it be the New Year red envelope given by the system?
But obviously the system cannot do this kind of thing.
Lu Liang looked through the sources of source points, and he quickly understood where these extra source points came from.
This was the source of sales for him after the pancreatic cancer drug was launched.
After figuring out the origin of the source point, Lu Liang once again set his sights on the system panel.
The analysis of this anti-β-amyloid antibody requires four source points. Among the drugs that Lu Liang has analyzed in the past, this can be regarded as a relatively high source point consumption.
Lu Liang didn't think much, he directly chose to analyze the medicine.
In an instant, Lu Liang felt a large amount of information pouring into Lu Liang's mind.
I don't know if it's because he has analyzed drugs more times, but Lu Liang has become more and more skilled in receiving this information.
After completing the reception of the information, Lu Liang created a new document on the computer desktop.
Because the drug is a monoclonal antibody drug and not a chemical molecule synthesis, the monoclonal antibody culture method must be used.
In addition, the most important thing is the experimental plan.
How should this kind of animal testing be conducted to evaluate the effects of neurological drugs?
In the animal tests of drugs conducted by Lu Liang in the past, the effect of the drugs could be judged basically through the expression of some proteins in cells and serum levels.
Because things like protein expression and serum levels are real and concrete, there is no possibility of deceiving people.
Therefore, when Lu Liang conducted experiments in the past, he only needed to use mice as the experimental model. After the experiment was completed, the mice were dissected and sampled.
But the problem is that in the articles Lu Liang read, the evaluation of whether drugs are effective for Alzheimer's disease is based on abstract concepts such as cognitive level, memory level, etc.
This made Lu Liang stunned for a moment. How to evaluate things like cognitive level? This is not like serum indicators, which can be detected by drawing a tube of blood.
After hesitating for a moment, Lu Liang opened the folder on the desktop again.
He found an experimental article about Alzheimer's disease and flipped through it to see other people's experimental methods.
The test subject used by the other party was still a mouse model, which made Lu Liang feel a little relieved.
He also thought that experiments related to the nervous system required primates as test subjects, such as monkeys.
The money spent is not that expensive.
Lu Liang remembered where he had seen a quote for a monkey, a primate experimental model, at 400,000 yuan.
Compared to this primate model, mice pale into insignificance.
Lu Liang continued to look down, but the next test method made Lu Liang silent again.
Labeling of mouse ET neurons via fMOST technology system combined with Fezf2-Cre.
Detection of ET neurons in the mPFC region of normal mice and AD model mice by fiber optic calcium imaging.
Immunofluorescence experiments label neuronal activity in brain areas such as the anterior olfactory nucleus, substantia innominate area, VTA, substantia nigra, and supramammillary nucleus.
emmm
Lu Liang had heard of the fMOST technology system, which was a whole-brain fine imaging technology system.
It is said that this technology can obtain single-cell whole-brain connection maps of mice, precise positioning of neural circuits in the whole brain, and long-range projection tracking of neurons.
It can even be used to change the neural and vascular networks of various model animals such as fruit flies, zebrafish, mice, rats, and primates during normal, disease, and development processes.
This technical system sounds very powerful.
But Lu Liang knows very well that in the field of scientific research, the more powerful the technology, it will definitely require extremely expensive equipment to support it.
If he remembered correctly, this thing was to be equipped with an optical tomography microscopy instrument.
After all, the upper limit of software is determined by hardware. You can't use a microscope to see the atomic structure or use a Nokia to play League of Legends, right?
Such a set of equipment and a system would probably cost less than 7 to 8 million.
Lu Liang looked helpless.
However, what Lu Liang cares about is not the price, because the price of the equipment is not the key to the problem.
If this kind of equipment will be used frequently in future tests, Lu Liang can definitely find a way to declare it, but if it doesn't work, he can go to the ancestor to find a way.
But the key point is that after he uses this instrument this time, he may not be able to use it again in the future.
Millions of instruments were sitting in the laboratory gathering dust. Lu Liangguang felt like he was losing money just thinking about it.
Moreover, the approval procedures for equipment applications are extremely troublesome, especially the purchase approval for such expensive equipment.
After a process is completed, I don’t even know that the Year of the Monkey and the Horse have passed.
Lu Liang thought for a moment and decided to think of other ways.
After hesitating for a long time, Lu Liang picked up his cell phone and dialed the number of Manager Nie of Hengfeng Biotechnology.
Generally speaking, biological companies still have a lot of high-precision equipment. Lu Liang planned to ask Manager Nie if there was such equipment.
Soon, Manager Nie answered the phone.
After listening to Lu Liang's needs, Manager Nie thought for a moment: Do you need an optical tomography microscopy instrument equipped with the fMOST technology system?
Yes, do you have Manager Nie over there? Lu Liang asked tentatively.
Manager Nie was silent for a while and then replied: I'm afraid our company does not have such equipment.
Lu Liang was a little disappointed: That's okay.
Manager Nie smiled helplessly: No way, Mr. Lu, it's not profitable to buy this kind of equipment.
When he said this, Manager Nie suddenly paused: However, I remember that there seems to be a domestic biological company that has this kind of equipment.
Hearing what Manager Nie said, Lu Liang's eyes lit up: Really? That's just right.
Manager Nie continued: However, Mr. Lu, do you really need it? Think carefully first. Using this kind of instrument to test samples is not cheap.
Lu Liangliang had a vague premonition: How much does his family quote for a sample?
When Lu Liangneng asked this question, he heard Manager Nie cough dryly on the other end of the phone: Actually, it's not very expensive, it's only about 50,000 yuan to test one sample.
Lu Liang was silent for a long time, and finally said one word.
6!
Early in the morning, Lu Liang got up from the bed. Because it was close to the start of school for college students, there were more and more people in the school visible to the naked eye.
Many students have arrived at school early, obviously they can no longer stay at home.
Unfortunately, the cafeteria is not open yet, so Lu Liang can only go to the breakfast shop outside the school to buy a pancake and fruit, and eat while walking towards the study room.
Wang Wentao had already arrived at the study room. As soon as Lu Liang opened the door of the study room, he saw Wang Wentao sitting in front of the computer and checking something.
It's rare to work so hard. When Lu Liang passed by Wang Wentao, he saw that Wang Wentao was reading literature.
Ever since he learned from Lu Liang that there was a way to alleviate Alzheimer's disease, Wang Wentao's whole body seemed to have been injected with chicken blood, and he changed from his previous decadence.
Wang Wentao said: There have been no experiments recently, so I can only find some and ask around.
Lu Liang walked over curiously and asked, So, did you see anything?
Wang Wentao scratched his head and said: I saw an article saying that it can promote the metabolism of tryptophan into indole acetic acid through the probiotics in the intestines.
Then indoleacetic acid can cross the blood-brain barrier, enter the brain tissue, improve the brain's immune microenvironment, inhibit neuroinflammation, and thereby repair neuronal function.
Lu Liang took a bite of the pancake fruit and said, It's quite novel, but it's probably difficult to apply it to people. The gut-brain axis is much more complicated than imagined.
After stuffing the pancake fruit into his mouth, Lu Liang threw the garbage into the trash can and turned on the computer.
Yesterday, he had already communicated with the biological company. 50,000 yuan per sample was actually within Lu Liang's acceptable range.
It's better to test two samples at most than to wait here all the time.
However, this should be the most expensive experiment in his history. It really is what he said, no money for scientific research, Lu Liang thought.
After concentrating, Lu Liang began to think about the omissions in the experiment.
The preparation method of the drug monoclonal antibody has also been sent to Tian Li. Now we just need to wait for Tian Li to send the drug sample before the test can begin.
During this time, Lu Liang planned to write a review.
I have read a lot of articles about Alzheimer's disease during this period, so it shouldn't be a problem to come up with a review, which can also provide me with some additional sources.
Lu Liang thought as he created a new word document.